Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one‐hybrid screens
Identifieur interne : 001463 ( Main/Exploration ); précédent : 001462; suivant : 001464Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one‐hybrid screens
Auteurs : Bat-Hen Ben Daniel [Israël] ; Esther Cattan [Israël] ; Chaim Wachtel [Israël] ; Dorit Avrahami [Israël] ; Yair Glick [Israël] ; Asaf Malichy [Israël] ; Doron Gerber [Israël] ; Gad Miller [Israël]Source :
- Physiologia Plantarum [ 0031-9317 ] ; 2016-08.
Abstract
To appropriately acclimate to environmental stresses, plants have to rapidly activate a specific transcriptional program. Yet, the identity and function of many of the transcriptional regulators that mediate early responses to abiotic stress stimuli is still unknown. In this work we employed the promoter of the multi‐stress‐responsive zinc‐finger protein Zat12 in yeast one‐hybrid (Y1H) screens to identify early abiotic stress‐responsive transcriptional regulators. Analysis of Zat12 promoter fragments fused to luciferase underlined an approximately 200 bp fragment responsive to NaCl and to reactive oxygen species (ROS). Using these segments and others as baits against Y1H control or stress Arabidopsis prey libraries, we identified 15 potential Zat12 transcriptional regulators. Among the prominent proteins identified were known transcription factors including bZIP29 and ANAC91 as well as unknown function proteins such as a homolog of the human USB1, a U6 small nuclear RNA (snRNA) processing protein, and dormancy/auxin‐associated family protein 2 (DRM2). Altered expression of Zat12 during high light stress in the knockout mutants further indicated the involvement of these proteins in the regulation of Zat12. Using a state of the art microfluidic approach we showed that AtUSB1 and DRM2 can specifically bind dsDNA and were able to identify the preferred DNA‐binding motif of all four proteins. Overall, the proteins identified in this work provide an important start point for charting the earliest signaling network of Zat12 and of other genes required for acclimation to abiotic stresses.
Url:
DOI: 10.1111/ppl.12439
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000337
- to stream Istex, to step Curation: 000337
- to stream Istex, to step Checkpoint: 000047
- to stream Main, to step Merge: 001468
- to stream Main, to step Curation: 001463
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one‐hybrid screens</title><author><name sortKey="Ben Daniel, Bat En" sort="Ben Daniel, Bat En" uniqKey="Ben Daniel B" first="Bat-Hen" last="Ben Daniel">Bat-Hen Ben Daniel</name></author><author><name sortKey="Cattan, Esther" sort="Cattan, Esther" uniqKey="Cattan E" first="Esther" last="Cattan">Esther Cattan</name></author><author><name sortKey="Wachtel, Chaim" sort="Wachtel, Chaim" uniqKey="Wachtel C" first="Chaim" last="Wachtel">Chaim Wachtel</name></author><author><name sortKey="Avrahami, Dorit" sort="Avrahami, Dorit" uniqKey="Avrahami D" first="Dorit" last="Avrahami">Dorit Avrahami</name></author><author><name sortKey="Glick, Yair" sort="Glick, Yair" uniqKey="Glick Y" first="Yair" last="Glick">Yair Glick</name></author><author><name sortKey="Malichy, Asaf" sort="Malichy, Asaf" uniqKey="Malichy A" first="Asaf" last="Malichy">Asaf Malichy</name></author><author><name sortKey="Gerber, Doron" sort="Gerber, Doron" uniqKey="Gerber D" first="Doron" last="Gerber">Doron Gerber</name></author><author><name sortKey="Miller, Gad" sort="Miller, Gad" uniqKey="Miller G" first="Gad" last="Miller">Gad Miller</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:547123E62951CF50536135668B13D3FBB809DFD7</idno><date when="2016" year="2016">2016</date><idno type="doi">10.1111/ppl.12439</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-NGPSLMR7-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000337</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000337</idno><idno type="wicri:Area/Istex/Curation">000337</idno><idno type="wicri:Area/Istex/Checkpoint">000047</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000047</idno><idno type="wicri:doubleKey">0031-9317:2016:Ben Daniel B:identification:of:novel</idno><idno type="wicri:Area/Main/Merge">001468</idno><idno type="wicri:Area/Main/Curation">001463</idno><idno type="wicri:Area/Main/Exploration">001463</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one‐hybrid screens</title><author><name sortKey="Ben Daniel, Bat En" sort="Ben Daniel, Bat En" uniqKey="Ben Daniel B" first="Bat-Hen" last="Ben Daniel">Bat-Hen Ben Daniel</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation></author><author><name sortKey="Cattan, Esther" sort="Cattan, Esther" uniqKey="Cattan E" first="Esther" last="Cattan">Esther Cattan</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation></author><author><name sortKey="Wachtel, Chaim" sort="Wachtel, Chaim" uniqKey="Wachtel C" first="Chaim" last="Wachtel">Chaim Wachtel</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation></author><author><name sortKey="Avrahami, Dorit" sort="Avrahami, Dorit" uniqKey="Avrahami D" first="Dorit" last="Avrahami">Dorit Avrahami</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Nanotechnology Institute, The Mina and Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat Gan</wicri:regionArea><wicri:noRegion>Ramat Gan</wicri:noRegion></affiliation></author><author><name sortKey="Glick, Yair" sort="Glick, Yair" uniqKey="Glick Y" first="Yair" last="Glick">Yair Glick</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Nanotechnology Institute, The Mina and Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat Gan</wicri:regionArea><wicri:noRegion>Ramat Gan</wicri:noRegion></affiliation></author><author><name sortKey="Malichy, Asaf" sort="Malichy, Asaf" uniqKey="Malichy A" first="Asaf" last="Malichy">Asaf Malichy</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Nanotechnology Institute, The Mina and Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat Gan</wicri:regionArea><wicri:noRegion>Ramat Gan</wicri:noRegion></affiliation></author><author><name sortKey="Gerber, Doron" sort="Gerber, Doron" uniqKey="Gerber D" first="Doron" last="Gerber">Doron Gerber</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Nanotechnology Institute, The Mina and Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat Gan</wicri:regionArea><wicri:noRegion>Ramat Gan</wicri:noRegion></affiliation></author><author><name sortKey="Miller, Gad" sort="Miller, Gad" uniqKey="Miller G" first="Gad" last="Miller">Gad Miller</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>The Mina & Everard Goodman Faculty of Life Sciences, Bar‐Ilan University, 5290002, Ramat‐Gan</wicri:regionArea><wicri:noRegion>Ramat‐Gan</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Israël</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Physiologia Plantarum</title><title level="j" type="alt">PHYSIOLOGIA PLANTARUM</title><idno type="ISSN">0031-9317</idno><idno type="eISSN">1399-3054</idno><imprint><biblScope unit="vol">157</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="422">422</biblScope><biblScope unit="page" to="441">441</biblScope><biblScope unit="page-count">20</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2016-08">2016-08</date></imprint><idno type="ISSN">0031-9317</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0031-9317</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">To appropriately acclimate to environmental stresses, plants have to rapidly activate a specific transcriptional program. Yet, the identity and function of many of the transcriptional regulators that mediate early responses to abiotic stress stimuli is still unknown. In this work we employed the promoter of the multi‐stress‐responsive zinc‐finger protein Zat12 in yeast one‐hybrid (Y1H) screens to identify early abiotic stress‐responsive transcriptional regulators. Analysis of Zat12 promoter fragments fused to luciferase underlined an approximately 200 bp fragment responsive to NaCl and to reactive oxygen species (ROS). Using these segments and others as baits against Y1H control or stress Arabidopsis prey libraries, we identified 15 potential Zat12 transcriptional regulators. Among the prominent proteins identified were known transcription factors including bZIP29 and ANAC91 as well as unknown function proteins such as a homolog of the human USB1, a U6 small nuclear RNA (snRNA) processing protein, and dormancy/auxin‐associated family protein 2 (DRM2). Altered expression of Zat12 during high light stress in the knockout mutants further indicated the involvement of these proteins in the regulation of Zat12. Using a state of the art microfluidic approach we showed that AtUSB1 and DRM2 can specifically bind dsDNA and were able to identify the preferred DNA‐binding motif of all four proteins. Overall, the proteins identified in this work provide an important start point for charting the earliest signaling network of Zat12 and of other genes required for acclimation to abiotic stresses.</div></front></TEI><affiliations><list><country><li>Israël</li></country></list><tree><country name="Israël"><noRegion><name sortKey="Ben Daniel, Bat En" sort="Ben Daniel, Bat En" uniqKey="Ben Daniel B" first="Bat-Hen" last="Ben Daniel">Bat-Hen Ben Daniel</name></noRegion><name sortKey="Avrahami, Dorit" sort="Avrahami, Dorit" uniqKey="Avrahami D" first="Dorit" last="Avrahami">Dorit Avrahami</name><name sortKey="Avrahami, Dorit" sort="Avrahami, Dorit" uniqKey="Avrahami D" first="Dorit" last="Avrahami">Dorit Avrahami</name><name sortKey="Cattan, Esther" sort="Cattan, Esther" uniqKey="Cattan E" first="Esther" last="Cattan">Esther Cattan</name><name sortKey="Gerber, Doron" sort="Gerber, Doron" uniqKey="Gerber D" first="Doron" last="Gerber">Doron Gerber</name><name sortKey="Gerber, Doron" sort="Gerber, Doron" uniqKey="Gerber D" first="Doron" last="Gerber">Doron Gerber</name><name sortKey="Glick, Yair" sort="Glick, Yair" uniqKey="Glick Y" first="Yair" last="Glick">Yair Glick</name><name sortKey="Glick, Yair" sort="Glick, Yair" uniqKey="Glick Y" first="Yair" last="Glick">Yair Glick</name><name sortKey="Malichy, Asaf" sort="Malichy, Asaf" uniqKey="Malichy A" first="Asaf" last="Malichy">Asaf Malichy</name><name sortKey="Malichy, Asaf" sort="Malichy, Asaf" uniqKey="Malichy A" first="Asaf" last="Malichy">Asaf Malichy</name><name sortKey="Miller, Gad" sort="Miller, Gad" uniqKey="Miller G" first="Gad" last="Miller">Gad Miller</name><name sortKey="Miller, Gad" sort="Miller, Gad" uniqKey="Miller G" first="Gad" last="Miller">Gad Miller</name><name sortKey="Wachtel, Chaim" sort="Wachtel, Chaim" uniqKey="Wachtel C" first="Chaim" last="Wachtel">Chaim Wachtel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001463 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001463 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:547123E62951CF50536135668B13D3FBB809DFD7
|texte= Identification of novel transcriptional regulators of Zat12 using comprehensive yeast one‐hybrid screens
}}
| This area was generated with Dilib version V0.6.33. |  |